| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045285 |
| Chromosome: | chromosome 13 |
| Location: | 2732738 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582476 | (1 of 1) K01112 - phosphohistidine phosphatase (PHPT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTACGGGAGAAGCCGCCGGGTGTGGAG |
| Internal bar code: | CGGTTAGCACTCGTAGTAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 97.366 |
| LEAP-Seq n confirming: | 1072 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACACACACACGATGCAC |
| Suggested primer 2: | AAAGTATAAGGGGCGGCTGT |