| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045287 |
| Chromosome: | chromosome_1 |
| Location: | 2012989 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g010832 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACGTGTGCATGTGCATGTAAACTACTTCC |
| Internal bar code: | TGATGGAGGTCGTAGGGTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 966 |
| LEAP-Seq percent confirming: | 99.3163 |
| LEAP-Seq n confirming: | 9297 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACATAAATTGCCACGACG |
| Suggested primer 2: | CAGGTAGAGAAGTGGGCGAG |