Insertion junction: LMJ.RY0402.045291_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.045291
Chromosome:chromosome 10
Location:4386766
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g451450 (1 of 66) 2.7.12.1 - Dual-specificity kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACGATGAGAGCGGTGAGTGAAGAGAGTT
Internal bar code:CGTACATTCCTCCTCTGCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:15
LEAP-Seq percent confirming:2.05128
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:573
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTTATTTCTCCTTGTGC
Suggested primer 2:AAAGTGAACTCGGGTTGGTG