| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045298 |
| Chromosome: | chromosome 13 |
| Location: | 916414 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567901 | (1 of 1) PTHR15451//PTHR15451:SF19 - FAMILY NOT NAMED // ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAAGGATTGCCAGCAAGGACGCAGGGC |
| Internal bar code: | AGCCCTACGGCTACCTCTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 99.4191 |
| LEAP-Seq n confirming: | 5819 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGGGACCTCACAACAT |
| Suggested primer 2: | GGTTTTGCCCCAATTCCTAT |