| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045308 |
| Chromosome: | chromosome 1 |
| Location: | 6257512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044800 | DGAT1,PFL1 | (1 of 1) 2.3.1.54 - Formate C-acetyltransferase / Pyruvate formate-lyase; Pyruvate-formate lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTGGCGAGGCCCCGGCGCATTGTCTTC |
| Internal bar code: | TCACGTCTAGCTGCACGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 99.6925 |
| LEAP-Seq n confirming: | 4214 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTACCGTATTCCCGGTG |
| Suggested primer 2: | GTCGGTTTGAGAGATTGGGA |