Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.045313 |
Chromosome: | chromosome_3 |
Location: | 5901071 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g190000 | PPP14 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGCACAGCACAGGCCGGTGGGCGGATGGT |
Internal bar code: | AGCTATGAATTTCGCATCGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 323 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 175 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCACTGAGCCAAAGAGAAC |
Suggested primer 2: | GCTCTCCGTAGATCTGGCAC |