| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045320 |
| Chromosome: | chromosome 4 |
| Location: | 2273921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220250 | (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCGCCACGCTCCCCGCCACGCCCCGC |
| Internal bar code: | GGCGGCCGGCTTGTGCGACGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 88.9785 |
| LEAP-Seq n confirming: | 331 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGTGTGGAGGAGGTTG |
| Suggested primer 2: | CAGCCTCATCTACAACACCG |