| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045333 |
| Chromosome: | chromosome 9 |
| Location: | 7002987 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410950 | NIA1,NIT1 | Nitrate reductase; (1 of 1) 1.6.2.2//1.7.1.1 - Cytochrome-b5 reductase // Nitrate reductase (NADH) / Nitrate reductase (NADH(2)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCCCCTTCCACACACGCACACACACA |
| Internal bar code: | TGACGGCTGTGTGCGTCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 92.2705 |
| LEAP-Seq n confirming: | 382 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATCAGGATGGACTCGG |
| Suggested primer 2: | CACGTGTGAGCGGCTAGTTA |