| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045347 |
| Chromosome: | chromosome 14 |
| Location: | 3947185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633100 | (1 of 1) PF12070 - Protein of unknown function (DUF3550/UPF0682) (DUF3550) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGAACGGCGGGGGCTACATGCCACAAG |
| Internal bar code: | GTCTGTATTACATTCACGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 99.6063 |
| LEAP-Seq n confirming: | 253 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAAGGAGTGTCTGTCGCA |
| Suggested primer 2: | TCTGCGTTCAGGTACACGAG |