Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.045354 |
Chromosome: | chromosome_7 |
Location: | 3631977 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g337000 | CSD3 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCCCTACTAACTCCAACAAACTCCCCCCC |
Internal bar code: | GCTACGCGGTCGCGCAGTGTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 534 |
LEAP-Seq percent confirming: | 93.6495 |
LEAP-Seq n confirming: | 1106 |
LEAP-Seq n nonconfirming: | 75 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCACATGAGGTGGTGTTG |
Suggested primer 2: | CGTGCACTAAGCAGGTCAAA |