| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045354 |
| Chromosome: | chromosome 7 |
| Location: | 3631977 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337000 | CSD3 | (1 of 2) 5.1.1.17 - Isopenicillin-N epimerase; Putative cysteine desulfurase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTACTAACTCCAACAAACTCCCCCCC |
| Internal bar code: | GCTACGCGGTCGCGCAGTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 93.6495 |
| LEAP-Seq n confirming: | 1106 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACATGAGGTGGTGTTG |
| Suggested primer 2: | CGTGCACTAAGCAGGTCAAA |