| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045358 |
| Chromosome: | chromosome 12 |
| Location: | 2583745 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505200 | HEL51 | (1 of 1) K14777 - ATP-dependent RNA helicase DDX47/RRP3 [EC:3.6.4.13] (DDX47, RRP3); DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGGCCACCATGCAAATGAAGGAGGCG |
| Internal bar code: | CACATTATTGGATTAGGATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 99.5124 |
| LEAP-Seq n confirming: | 28571 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGCCCCGCAAACTTGTA |
| Suggested primer 2: | GTGCGTGCAGTATAGCGTGT |