| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045378 |
| Chromosome: | chromosome 17 |
| Location: | 5654253 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTATTATAACTCATAAGGTGACACTTT |
| Internal bar code: | AACGGAGAGCAAAATGCGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 97.2638 |
| LEAP-Seq n confirming: | 1493 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCCTATAGCGACGAGCC |
| Suggested primer 2: | CTCTGGCTGCCCTAGTTTTG |