Insertion junction: LMJ.RY0402.045381_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.045381
Chromosome:chromosome 6
Location:5545152
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g285200 NDH1 Nucleoside diphosphate hydrolase; (1 of 1) 3.6.1.13//3.6.1.18 - ADP-ribose diphosphatase / ADPR-PPase // FAD diphosphatase / FAD pyrophosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGTCACGTTCGTCAGAGGAGATGGCTC
Internal bar code:ATTTCACGAAGGCTCCGATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:98.7856
LEAP-Seq n confirming:3091
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AATAGCGGAGGTGACGCTAA
Suggested primer 2:CTGCACCACCAAGCTTATGA