| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045389 |
| Chromosome: | chromosome 4 |
| Location: | 2620752 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222800 | LCID,LCID1 | Low-CO2 inducible protein D | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTGGGTCAGGTTGGCCTCCAGCGCCGA |
| Internal bar code: | TTGTGTATAAAGGGCGTCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 99.8448 |
| LEAP-Seq n confirming: | 2573 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGATTGCTCACCATGAC |
| Suggested primer 2: | GAATACGTAGCGCTCCTTGC |