| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045392 |
| Chromosome: | chromosome 17 |
| Location: | 39432 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696350 | (1 of 1) PTHR12454:SF11 - PROTEIN Y57A10A.10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTTACAAGCAAGGGAGTGGGCAAGTGA |
| Internal bar code: | ACATCCGTGGATCCGAAAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 99.5825 |
| LEAP-Seq n confirming: | 954 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGCCCTTTTGTTAGTGT |
| Suggested primer 2: | CAAAAGGGAGGGTTTGTTGA |