Insertion junction: LMJ.RY0402.045393_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.045393
Chromosome:chromosome 2
Location:3501854
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095125 (1 of 1) IPR000104//IPR000408//IPR009091 - Antifreeze protein, type I // Regulator of chromosome condensation, RCC1 // Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGATCTTGGAAGCCCTTGAGCCTTGTTC
Internal bar code:GTGGCACTCAGTGATGGGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:647
LEAP-Seq percent confirming:91.221
LEAP-Seq n confirming:26164
LEAP-Seq n nonconfirming:2518
LEAP-Seq n unique pos:107

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACGTCG
Suggested primer 2:GTGTCACGAGCAGGGAAAAT