| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045401 |
| Chromosome: | chromosome 8 |
| Location: | 3347931 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374150 | (1 of 11) IPR011146 - HIT-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACGCACGTGAGGCGACACGCAATCGGA |
| Internal bar code: | CTAGCAATAGGTAGATGCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 99.536 |
| LEAP-Seq n confirming: | 429 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCATGGGTTGTTAAGACT |
| Suggested primer 2: | AGTTGCTGCCTGTGTGTCTG |