| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045411 |
| Chromosome: | chromosome 12 |
| Location: | 1414842 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488000 | FFT4 | putative fructan fructosyltransferase; (1 of 2) 2.4.1.99 - Sucrose:sucrose fructosyltransferase / Sucrose:sucrose 1-fructosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTCCTGCAACATGCAATGTGGGTCTTA |
| Internal bar code: | GAATTTACCGGAAGTTACTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 99.7072 |
| LEAP-Seq n confirming: | 2043 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCACTCTCCCTGACTGC |
| Suggested primer 2: | CAAGGCTCTTACAACGCACA |