Insertion junction: LMJ.RY0402.045420_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.045420
Chromosome:chromosome 2
Location:4996145
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g104201 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCAGCCCACTTTTCCCTTGTCCCAGGGG
Internal bar code:TAGGCTCGCTCTTATCTACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1267
LEAP-Seq percent confirming:99.1632
LEAP-Seq n confirming:5214
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCACGTGAGCTTCCTCGT
Suggested primer 2:CACCATCCCCACTAATCCAC