| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045448 |
| Chromosome: | chromosome 10 |
| Location: | 3713045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446250 | (1 of 1) K13339 - peroxin-6 (PEX6, PXAAA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCCAAGCAGTGCATCAAAGCGGGCATA |
| Internal bar code: | GCGCGGCAACCGTGGTAGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 99.3809 |
| LEAP-Seq n confirming: | 4816 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTACCGCCACGGATACTA |
| Suggested primer 2: | ATCGGGATTGAAGACGACTG |