| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045463 |
| Chromosome: | chromosome 1 |
| Location: | 3421300 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021850 | HEL2 | (1 of 2) PTHR24031:SF249 - ATP-DEPENDENT DNA HELICASE RECG; DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCAAGAACAAGTTGAGGGCTTGAACC |
| Internal bar code: | GGGGTAGAGATTTGGTGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 95.4095 |
| LEAP-Seq n confirming: | 4240 |
| LEAP-Seq n nonconfirming: | 204 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGCAGTAGCGCCGTTGT |
| Suggested primer 2: | ACATGACACCTCGTGCACAT |