Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.045494 |
Chromosome: | chromosome 10 |
Location: | 1843732 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g431250 | CYG5 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCCTCGTTCAAACCGCAGTGTGGCACA |
Internal bar code: | GCATCAGCCGCACACACGTGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 913 |
LEAP-Seq percent confirming: | 98.2262 |
LEAP-Seq n confirming: | 886 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTGTGCTTCCCTGATACCG |
Suggested primer 2: | TGCTGTTGTAGAGGGAGCCT |