Insertion junction: LMJ.RY0402.045532_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.045532
Chromosome:chromosome 3
Location:4205234
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g174050 MRP4 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTCGGTGCGCTGTGGCTGACCCTGACCC
Internal bar code:AACGCTGCCGCCAGTGGCTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:94.7009
LEAP-Seq n confirming:59868
LEAP-Seq n nonconfirming:3350
LEAP-Seq n unique pos:134

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCGCCTTCATCTTACCT
Suggested primer 2:GTCCATGTGCGAAAACACAC