| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045565 |
| Chromosome: | chromosome 6 |
| Location: | 1543627 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCACTCGCCTCCAAACTCGCGTCCTGTT |
| Internal bar code: | TACTTTAGTGAAGATGCCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 99.4947 |
| LEAP-Seq n confirming: | 4923 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTAGCATTTGGGTGAGTT |
| Suggested primer 2: | AGGCATTTCACAGAACACCC |