| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045567 |
| Chromosome: | chromosome 1 |
| Location: | 4764507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACCTCCCTCAATTATGCTCGATGATCT |
| Internal bar code: | GCGGCGATAGGATAACGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 95.5049 |
| LEAP-Seq n confirming: | 1551 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGAGTCCTAGCCAAGCC |
| Suggested primer 2: | AGGGATAACAAACAGCACGG |