| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045569 |
| Chromosome: | chromosome 6 |
| Location: | 3897363 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278180 | 5'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGCGGCATGATAGCGTACAGGCGCAC |
| Internal bar code: | TAGCCAACTAGGTTTTTCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 97.0646 |
| LEAP-Seq n confirming: | 1488 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGTTCTCCAGGTTGGT |
| Suggested primer 2: | TCCGGTGGACTCTCCTACTG |