| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045571 |
| Chromosome: | chromosome 16 |
| Location: | 2074191 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657400 | (1 of 62) PF00226 - DnaJ domain (DnaJ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTCCCTGTCTCGCTCCTCCTGCTCTG |
| Internal bar code: | ACGATCGGGCGTCTCGTCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTTCGGTGTTGTCCTG |
| Suggested primer 2: | CATCCTCTTCTGCGTGTCAA |