Insertion junction: LMJ.RY0402.045586_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.045586
Chromosome:chromosome 1
Location:4448692
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g030200 PP2C4,PPP3,FAP6 Flagellar Associated Protein 6; (1 of 1) PTHR13832:SF273 - PROTEIN TAP-1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCACGCTTTATTACCCGAGGTGGACCCG
Internal bar code:GTGGGTATCTGCCCTTCTGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:897
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGTTTGGGAGTTCATCT
Suggested primer 2:TCTGGTTAGCGGTCTTTGCT