Insertion junction: LMJ.RY0402.045610_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.045610
Chromosome:chromosome 4
Location:2889569
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224500 (1 of 1) 3.6.3.17//3.6.3.44 - Monosaccharide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCTGGCGTACTGGCGCTCGCCGGGCTAC
Internal bar code:TGTCGATATTCCTATGGAAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:99.5607
LEAP-Seq n confirming:8385
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCAAAAGCCCTGTTACG
Suggested primer 2:TTCGAGTCCTTCGACCAGTT