Insertion junction: LMJ.RY0402.045615_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.045615
Chromosome:chromosome 3
Location:5365540
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g184400 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase; Putative NAD+ diphosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCGCCAAATGTTCACCCCATGATTCAA
Internal bar code:CCCCCACCGACATGGGTACCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:797
LEAP-Seq percent confirming:73.9889
LEAP-Seq n confirming:32800
LEAP-Seq n nonconfirming:11531
LEAP-Seq n unique pos:96

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGACTACGCTGGGTCAT
Suggested primer 2:GGGGTTTCTGCGTTTTTGTA