| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045620 |
| Chromosome: | scaffold_19 |
| Location: | 61015 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre19.g750447 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAAGGGGTGCGGACGTTCGGTGGTGGGCT |
| Internal bar code: | GGGCCTAGTACGGGCCTGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 95.3299 |
| LEAP-Seq n confirming: | 1878 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGACTTTGTGTGGTTA |
| Suggested primer 2: | TGCAAGTCAAGATGTGCCTC |