| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045624 |
| Chromosome: | chromosome_8 |
| Location: | 91381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g358528 | FAP157 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTTCGAGGTCGTCAACTGCGCAGCCTTGA |
| Internal bar code: | TTGTATGACGCCATAGGCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 87.9908 |
| LEAP-Seq n confirming: | 381 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCGTAGTACGCGTGTTG |
| Suggested primer 2: | TCCTGGTACCTGTGAAAGGC |