| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045639 |
| Chromosome: | scaffold_19 |
| Location: | 51346 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre19.g750447 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATGTGTATACGGTACTCATGGTGCGGGCC |
| Internal bar code: | GACCCCGTCATATCATTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 99.7594 |
| LEAP-Seq n confirming: | 4560 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAACTCTCACACCCAACC |
| Suggested primer 2: | COULD_NOT_FIND_PRIMER |