| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045645 |
| Chromosome: | chromosome 9 |
| Location: | 3078880 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387050 | FRK1 | (1 of 1) 2.7.1.45 - 2-dehydro-3-deoxygluconokinase / KDG kinase; Putative fructokinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTAGCAAGCCCCAGCCGCGCCGCAGC |
| Internal bar code: | TCGTTTCTCCTGTCCGTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 68.4746 |
| LEAP-Seq n confirming: | 606 |
| LEAP-Seq n nonconfirming: | 279 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGCTGTCTTAATGCACG |
| Suggested primer 2: | AGATGAAGCCGTTGGTGAAG |