| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045645 |
| Chromosome: | chromosome 9 |
| Location: | 3078883 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387050 | FRK1 | (1 of 1) 2.7.1.45 - 2-dehydro-3-deoxygluconokinase / KDG kinase; Putative fructokinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCCACATCGGCACAGACATTTACGGC |
| Internal bar code: | GAGTCGGGGCCGTCGAAGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 96.3373 |
| LEAP-Seq n confirming: | 2262 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGCTGTCTTAATGCACG |
| Suggested primer 2: | AGATGAAGCCGTTGGTGAAG |