| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045656 |
| Chromosome: | chromosome_17 |
| Location: | 335018 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g698365 | FAP172 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCTCGCAGCAAGCAAAAGCGCGGCGAGAA |
| Internal bar code: | GGCGATTCATAGCTAGTCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 98.4074 |
| LEAP-Seq n confirming: | 2966 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGCTTGGCACAATAACC |
| Suggested primer 2: | TCCTCCAGTGACAACTGCTG |