| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045656 |
| Chromosome: | chromosome 17 |
| Location: | 335028 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698365 | PF7,CCDC40,FAP172 | Flagellar Associated Protein 172 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAGCCCGTAAGCGTGCGAGCGCCGTG |
| Internal bar code: | GGGCCACCCTACGTCTGAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.4148 |
| LEAP-Seq n confirming: | 1529 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGCTTGGCACAATAACC |
| Suggested primer 2: | TCCTCCAGTGACAACTGCTG |