Insertion junction: LMJ.RY0402.045681_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.045681
Chromosome:chromosome_16
Location:80922
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g695700 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GACTTGCTGCCGTCCTCCGCGGCGTTGATG
Internal bar code:TATGGTGTATCATGATTCAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:590
LEAP-Seq percent confirming:99.7625
LEAP-Seq n confirming:1260
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATGGTAAGGCACCGAAG
Suggested primer 2:CTCTCGGCGAGCTCTACACT