| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045706 |
| Chromosome: | chromosome 4 |
| Location: | 3288682 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226850 | ASP1 | (1 of 1) 3.4.23.5 - Cathepsin D; Pepsin-type aspartyl protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGCATATCAACGTGCAAGTACTGAAT |
| Internal bar code: | GGCTCGGACCGGCGGGAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 99.8621 |
| LEAP-Seq n confirming: | 1448 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAACATCCCGACAGGAC |
| Suggested primer 2: | TAGCATCAAACTGCTCGGTG |