| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045706 |
| Chromosome: | chromosome 4 |
| Location: | 3288695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226850 | ASP1 | (1 of 1) 3.4.23.5 - Cathepsin D; Pepsin-type aspartyl protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGGACCGGCTTCAACACCCACGAGTCGT |
| Internal bar code: | ATCGTGGCATGCTAATCGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 99.5968 |
| LEAP-Seq n confirming: | 494 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAACATCCCGACAGGAC |
| Suggested primer 2: | TAGCATCAAACTGCTCGGTG |