| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045716 |
| Chromosome: | chromosome 5 |
| Location: | 1761638 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242501 | EEP2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGTGGAGAACCCGGCGCTAGAAAGCTC |
| Internal bar code: | CGCAGTGTCGCGAACAGGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 64.1517 |
| LEAP-Seq n confirming: | 3282 |
| LEAP-Seq n nonconfirming: | 1834 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCCTCATCCACGACTAT |
| Suggested primer 2: | CTCACTAGCTGCATCCACCA |