| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045719 |
| Chromosome: | chromosome 1 |
| Location: | 2397630 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013250 | (1 of 2) IPR006357//IPR023214 - HAD-superfamily hydrolase, subfamily IIA // HAD-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGGCTGGCGGCGGCAGTAGGCATGAC |
| Internal bar code: | GGCGGTCTAGTCGGCAGTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGTATTTGCCCGTCTGC |
| Suggested primer 2: | GGCTTTAGAATGCGCGTTAG |