| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045752 |
| Chromosome: | chromosome 7 |
| Location: | 4127047 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342800 | CGL16 | (1 of 1) PF07063 - Domain of unknown function (DUF1338) (DUF1338); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCAGCAGCCCATAGGTGGGTGTTCTTA |
| Internal bar code: | GTGAGGCGGCTCCCTGTCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.5869 |
| LEAP-Seq n confirming: | 3375 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGAGCGAGATACAGGTG |
| Suggested primer 2: | TGGTTCAGTGAGTAGCCGTG |