| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045758 |
| Chromosome: | chromosome 6 |
| Location: | 1951507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263750 | RPN15 | component of lid subcomplex of 26S proteasome regulatory subunit; (1 of 2) PF05160 - DSS1/SEM1 family (DSS1_SEM1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCGTGGCAGGGATCTGAACACCTGGT |
| Internal bar code: | GAGGGCTCTGTCTCGTAGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 99.1961 |
| LEAP-Seq n confirming: | 18755 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCTGAGAGTCTAGGCG |
| Suggested primer 2: | GCAGGAGCGAGATGAGGTAG |