| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045768 |
| Chromosome: | chromosome 3 |
| Location: | 7236535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207700 | FPPS1,FPPS,PPS1,FPS1 | (1 of 1) 2.5.1.1//2.5.1.10 - Dimethylallyltranstransferase / Prenyltransferase // (2E,6E)-farnesyl diphosphate synthase / Geranyltranstransferase; Farnesyl pyrophosphate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGGTCGGCCGGACCTGCTTGCCACACT |
| Internal bar code: | CTTAGCGCGGTGACATTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 96.1512 |
| LEAP-Seq n confirming: | 1399 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGAGAGATAGGGAGGGC |
| Suggested primer 2: | GGCAGTTGCCTACGGTATGT |