| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045776 |
| Chromosome: | chromosome 12 |
| Location: | 8543072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547651 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTGGCAGGCTGCAGCGGGCGAGGCTCA |
| Internal bar code: | GTCGAAAAAAATGTCTGGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 76.4463 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCTCCTCGTCTTCGTCA |
| Suggested primer 2: | GAGTTGACGGAGACGAGGAG |