Insertion junction: LMJ.RY0402.045780_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.045780
Chromosome:chromosome 11
Location:2984871
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g478300 SOM4 S-adenosyl-L-methionine-dependent O-methyltransferase; (1 of 4) PTHR13600//PTHR13600:SF12 - LEUCINE CARBOXYL METHYLTRANSFERASE // LEUCINE CARBOXYL METHYLTRANSFERASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGGGGTGGCGGTGGCGGTCGCGTGGGGA
Internal bar code:CAGCCGGTTATCGCAGTAGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:60.1458
LEAP-Seq n confirming:60897
LEAP-Seq n nonconfirming:40352
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCAAACTAAACCAAA
Suggested primer 2:AGGTAGGAATGGGGGAAATG