Insertion junction: LMJ.RY0402.045782_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.045782
Chromosome:chromosome 15
Location:258157
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g640800 (1 of 1) PF15963 - Myb DNA-binding like (Myb_DNA-bind_7) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTGACACGCGGCTGGCTGGGCGGTGGC
Internal bar code:GGGCGCGTGATTTTTGAATAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:99.1525
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGTCGTCGTCCTCATCC
Suggested primer 2:GTAGGCTGCGATTCAGGAAG