| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045789 |
| Chromosome: | chromosome 8 |
| Location: | 12134 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358200 | ACV4 | protein kinase; (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCGATAACCACACGGGGGCGGCAGGC |
| Internal bar code: | TGTGCCCTTGATGGTTCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.4803 |
| LEAP-Seq n confirming: | 1340 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCTTTAGCAGTAGCGG |
| Suggested primer 2: | GAGGGGTTGCAGAGAGATTG |