| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045812 |
| Chromosome: | chromosome 13 |
| Location: | 4206174 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592400 | LCI21 | Low-CO2-inducible protein 21 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCAGTCCCCGCTTTCACGCCCAAGGGC |
| Internal bar code: | AACGCGACCGCTGATGTTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.6805 |
| LEAP-Seq n confirming: | 624 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTGCTATTACTGCGGGG |
| Suggested primer 2: | AGCTTTGCTGCCTGAAATGT |